Biotechnological aspects of sawflies number control in blackcurrant plantations

Gulaiym Mamyrova, Zhoomart Moldaliev, Merivan Kaarova, Yuliia Kolomiiets, Artem Kalinichenko
Received: 10.10.2024 Revised: 19.04.2025 Accepted: 28.05.2025
Retrieved from Vol. 28, No. 6, 2025 Pages: 65-76
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Abstract

The study aimed to compare the effectiveness of biotechnological methods of controlling the Pristiphora rufipes sawfly population on black currant (Ribes nigrum) in the forest-steppe zone of Ukraine and to identify environmental factors affecting effectiveness. The study was conducted using 20 plots (0.1 hectares each) divided into four groups: treatment with the biological product Bacillus thuringiensis, entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae), a combination of fungi with pheromone traps and control without treatment. The effectiveness was assessed through weekly monitoring of pest numbers, the degree of leaf damage according to the scale of the European and Mediterranean Plant Protection Organisations, and analysis of climatic parameters. The integrated group demonstrated the highest efficiency: the number of larvae decreased by 85±4% (p<0.001), adults by 60±6%, and the average score of leaf damage was 1.2 ± 0.1 against 4.1 ± 0.3 in the control. The Bacillus thuringiensis group showed a 72 ± 5% reduction in larvae (p < 0.001) with stability to temperatures of 15-25°C, but the effectiveness dropped to 65% after 20 days. The fungi reduced the population by 65 ± 7% (p75%), but effect was halved in the drought. The control group recorded a population growth of 25 ± 3%. Regression analysis showed that the integrated approach explained 89% of the variance (R2 = 0.89) in pest reduction. A comparative analysis with the Kyrgyzstan Ribes species (Ribes meyeri, Ribes saxatile) indicated the potential of using phytochemical adaptations for breeding resistant varieties. The results demonstrated that the combination of biological products with pheromones is the optimal strategy to reduce dependence on chemical insecticides in the forest-steppe zone of Ukraine, incorporating local climatic conditions (temperature, humidity) and biological characteristics of the pest. The study has practical implications for the development of sustainable berry crop protection systems

Keywords

pests; larvae; imago; pheromone traps; entomopathogenic fungi

  1. Achari, T.S., Panda, C., & Barik, T.K. (2022). Biochemical response of Aedes aegypti and Aedes albopictus mosquitoes after exposure to thermal stress and toxin of Bacillus thuringiensis israelensis. International Journal of Tropical Insect Science, 42, 651-660. doi: 10.1007/s42690-021-00587-4.
  2. Aguilar-Marcelino, L., Mendoza-de-Gives, P., Al-Ani, L.K., López-Arellano, M.E., Gómez-Rodríguez, O., Villar-Luna, E., & Reyes-Guerrero, D.E. (2020). Using molecular techniques applied to beneficial microorganisms as biotechnological tools for controlling agricultural plant pathogens and pest. In V. Sharma, R. Salwan & L.K. Tawfeeq Al-Ani (Eds.), Molecular aspects of plant beneficial microbes in agriculture (pp. 333-349). London: Academic Press. doi: 10.1016/B978-0-12-818469-1.00027-4.
  3. Alemu, M. (2020). Trend of biotechnology applications in pest management: A review. International Journal of Applied Sciences and Biotechnology, 8(2), 108-131. doi: 10.3126/ijasbt.v8i2.28326.
  4. Arthurs, S.P., & Lacey, L.A. (2024). Microbial control of insect pests of tree fruit. In K.G. Mukerji & G. Saxena (Eds.), Management of nematode and insect-borne diseases (pp. 1-46). Boca Raton: CRC Press. doi: 10.1201/9781003578314.
  5. Askary, H., Ghaffari, S., Asgari, M., & Karimi, J. (2021). Fungal entomopathogens of order hypocreales. In J. Karimi & H. Madadi (Eds.), Biological control of insect and mite pests in Iran: A review from fundamental and applied aspects (pp. 439-480). Cham: Springer. doi: 10.1007/978-3-030-63990-7_11.
  6. Bate-Smith, E.C. (1976). Chemistry and taxonomy of Ribes. Biochemical Systematics and Ecology, 4(1), 13-23. doi: 10.1016/0305-1978(76)90004-1.
  7. Bilyk, M.O. (2022). Biological protection of plants from pests. Kharkiv: Maidan.
  8. Botha, A.M., Kunert, K.J., Maling’a, J., & Foyer, C.H. (2020). Defining biotechnological solutions for insect control in sub-Saharan Africa. Food and Energy Security, 9(1), article number e191. doi: 10.1002/fes3.191.
  9. Chakravarthy, A.K. (2020). Innovative pest management approaches for the 21st century. Singapore: Springer. doi: 10.1007/978-981-15-0794-6.
  10. Devi, G. (2020). Entomopathogenic nematode against foliar pests. International Journal of Agriculture, Environment and Bioresearch, 5(3), 252-275. doi: 10.35410/IJAEB.2020.5520.
  11. European and Mediterranean Plant Protection Organization. (2021). Standards. Retrieved from https://gd.eppo. int/download/standard/91/pm4-012-1-en.pdf.
  12. Gołębiowski, M., Bojke, A., & Tkaczuk, C. (2021). Effects of the entomopathogenic fungi Metarhizium robertsii, Metarhizium flavoviride, and Isaria fumosorosea on the lipid composition of Galleria mellonella larvae. Mycologia, 113(3), 525-535. doi: 10.1080/00275514.2021.1877520.
  13. Guignard, Q., Bouwer, M., Slippers, B., & Allison, J. (2020). Biology of a putative male aggregation-sex pheromone in Sirex noctilio (Hymenoptera: Siricidae). PLoS One, 15(12), article number e0244943. doi: 10.1371/journal. pone.0244943.
  14. Hellmann, C., Greiner, A., & Vilcinskas, A. (2024). Design of polymer carriers for optimized pheromone release in sustainable insect control strategies. Advanced Science, 11(9), article number 2304098. doi: 10.1002/ advs.202304098.
  15. Hernández-Rosas, F., Figueroa-Rodríguez, K.A., García-Pacheco, L.A., Velasco-Velasco, J., & SangermanJarquín, D.M. (2020). Microorganisms and biological pest control: An analysis based on a bibliometric review. Agronomy, 10(11), article number 1808. doi: 10.3390/agronomy10111808.
  16. Honenko, L.G. (2021). Biological methods of protection of agroecosystems. Mykolaiv: Mykolaiv National Agrarian University.
  17. Hradchenko, S., & Pikovskyi, M. (2023). Efficiency of biological preparations against scab and powdery mildew of apple trees. Plant and Soil Science, 14(4), 76-85. doi: 10.31548/plant4.2023.76.
  18. Kalykova, G.N., Kupsuralieva, I.K., & Sagitov, A.O. (2022). Pests and diseases of Semyonov firs in Kyrgyzstan. Reports of the National Academy of Sciences of the Republic of Kazakhstan, 1(341), 21-26. doi: 10.32014/2022.25181483.128%20.
  19. Knapp, M., Palevsky, E., & Rapisarda, C. (2020). Insect and mite pests. In M.L. Gullino, R. Albajes & P.C. Nicot (Eds.), Integrated pest and disease management in greenhouse crops (pp. 101-146). Cham: Springer. doi: 10.1007/9783-030-22304-5_4.
  20. Kumari, P., Jasrotia, P., Kumar, D., Kashyap, P.L., Kumar, S., Mishra, C.N., Kumar, S., & Singh, G.P. (2022). Biotechnological approaches for host plant resistance to insect pests. Frontiers in Genetics, 13, article number 914029. doi: 10.3389/fgene.2022.914029.
  21. Kushnir, N., Bondareva, L., & Zavadska, O. (2023). Research of trophic connections and phenology Metcalfa pruinosa Say (Hemiptera: Flatidae) in the M. Gryshko National Botanical Garden of the NAS of Ukraine. Biological Systems: Theory and Innovation, 14(1), 101-111. doi: 10.31548/biologiya14(1-2).2023.001.
  22. Lampasona, T.P. (2022). Host associated behavior, ecology, and fitness of the plum Curculio conotrachelus nenuphar (Coleoptera: Curculionidae) in peach and blueberry. New Brunswick: State University of New Jersey. doi: doi:10.7282/t3-56bm-pf67.
  23. Meng, H.-H., Gao, X.-Y., Huang, J.-F., & Zhang, M.-L. (2015). Plant phylogeography in arid Northwest China: Retrospectives and perspectives. Journal of Systematics and Evolution, 53(1), 33-46. doi: 10.1111/jse.12088.
  24. Mushtruk, N., & Mushtruk, M. (2023). Analysis of the raw material base for pectin production. Animal Science and Food Technology, 14(2), 57-75. doi: 10.31548/animal.2.2023.57.
  25. Nagy, A., Szarukán, I., Szalárdi, T., Szanyi, S., Jósvai, J.K., & Tóth, M. (2022). Addition of 4-oxoisophorone improves performance of bisexual lure for Autographa gamma (L.) (Lepidoptera: Noctuidae). Journal of Applied Entomology, 146(3), 328-334. doi: 10.1111/jen.12958.
  26. Pacheco, S., Gómez, I., Soberón, M., & Bravo, A. (2023). A major conformational change of N-terminal helices of Bacillus thuringiensis Cry1Ab insecticidal protein is necessary for membrane insertion and toxicity. The FEBS Journal, 290(10), 2692-2705. doi: 10.1111/febs.16710.
  27. Parajuli, S., Shrestha, J., Subedi, S., & Pandey, M. (2022). Biopesticides: A sustainable approach for pest management: Biopesticides in sustainable pest management. SAARC Journal of Agriculture, 20(1), 1-13. doi: 10.3329/sja.v20i1.60526.
  28. Patyka, Т.І., & Patyka, М.V. (2020). Bacillus thuringiensis ssp. Israelensis and control of aedes aegypti invasive mosquitoes species in ecosystems. Microbiological Journal/Mikrobiolohichnyi Zhurnal, 82(5), 88-97. doi: 10.15407/ microbiolj82.05.088.
  29. Quesada-Moraga, E., Yousef-Naef, M., & Garrido-Jurado, I. (2020). Advances in the use of entomopathogenic fungi as biopesticides in suppressing crop pests. In N. Birch & T. Glare (Eds.), Biopesticides for sustainable agriculture (pp. 63-98). London: Burleigh Dodds Science Publishing. doi: 10.1201/9781003048008.
  30. Regulation of the European Parliament and of the Council No. 1107/2009 “Concerning the Placing of Plant Protection Products on the Market and Repealing Council Directives 79/117/EEC and 91/414/EEC”. (2009, October). Retrieved from https://www.kmu.gov.ua/storage/app/sites/1/55-GOEEI/es-11072009.pdf.
  31. Shahini, S., Mustafaj, S., Sula, U., Shahini, E., Skura, E., & Sallaku, F. (2023). Biological control of greenhouse whitefly Trialeurodes vaporariorum with Encarsia formosa: Special case developed in Albania. Evergreen, 10(4), 2084-2091. doi: 10.5109/7160868.
  32. Shahini, S., Skura, E., Huqi, A., Shahini, E., Ramadhi, A., & Sallaku, F. (2024). Integrated management of the mediterranean fruit fly (Ceratitis capitata) on citrus in the Konispol, Albania. Grassroots Journal of Natural Resources, 7(2), 324-346. doi: 10.33002/nr2581.6853.070217.
  33. Singh, S., Singh, A., Kumar, S., Mittal, P., & Singh, I.K. (2020). Protease inhibitors: Recent advancement in its usage as a potential biocontrol agent for insect pest management. Insect Science, 27(2), 186-201. doi: 10.1111/17447917.12641.
  34. Skliar, V., Smoliar, N., Kozak, M., Liubynskyi, O., & Skliar, Y. (2024). Ecological and cenotic features of natural regeneration of forests in the Left-Bank Polissya of Ukraine. Ukrainian Journal of Forest and Wood Science, 15(2), 118-134. doi: 10.31548/forest/2.2024.118.
  35. Strand, J.R., Perez Moya, O.G., Peterson, R.K., Sterling, T.M., & Weaver, D.K. (2025). Sensory and behavioral responses of braconid parasitoids to changes in volatile emissions induced by wheat stem sawfly (Hymenoptera: Cephidae) larval feeding in winter wheat and smooth brome. Journal of Insect Science, 25(2), article number 2. doi: 10.1093/jisesa/ieaf016.
  36. Sun, Q., Wang, N., Xu, W., & Zhou, H. (2021). Ribes himalense as potential source of natural bioactive compounds: Nutritional, phytochemical, and antioxidant properties. Food Science & Nutrition, 9(6), 2968-2984. doi: 10.1002/ fsn3.2256.
  37. Svensson, G.P., Anderbrant, O., Öberg, E., Jirle, E.V., Hellqvist, S., & Löfstedt, C. (2023). Identification of (E)-and (Z)11-tetradecenyl acetate as sex pheromone components of the currant pest Euhyponomeutoides albithoracellus. Journal of Applied Entomology, 147(5), 313-319. doi: 10.1111/jen.13115.
  38. Thakur, N., Tomar, P., Kaur, S., Jhamta, S., Thakur, R., & Yadav, A.N. (2021). Entomopathogenic soil microbes for sustainable crop protection. In A.N. Yadav (Ed.), Soil microbiomes for sustainable agriculture: Functional annotation (pp. 529-571). Cham: Springer. doi: 10.1007/978-3-030-73507-4_17.
  39. Tumenbayeva, N., Mombayeva, B., Kushenbekova, A., Kaliyeva, L., Mussina, M., Nurgaliyeva, G., Sarsengaliyev, R., & Sarmanova, R. (2024). Biological protection of black currant from phytophages. Caspian Journal of Environmental Sciences, 22(5), 1285-1291.
  40. Uma, G.S., Godara, S., Ramakrishnan, A., Nandini, J., & Kattappanavar, A.D. (2025). Eco-tech strategies: Revolutionizing forest insect pest control through biological and biotechnological innovations. In R.K. Kalia & R. Pathak (Eds.), Tree biology and biotechnology (pp. 233-259). Singapore: Springer. doi: 10.1007/978-981-960002-1_15.
  41. Xu, L., Xie, Y., Na, R., & Li, Q.X. (2020). Mini-review: Recent advances in the identification and application of sex pheromones of gall midges (Diptera: Cecidomyiidae). Pest Management Science, 76(12), 3905-3910. doi: 10.1002/ps.5949.
Mamyrova, G., Moldaliev, Zh., Kaarova, M., Kolomiiets, Yu., & Kalinichenko, A. (2025). Biotechnological aspects of sawflies number control in blackcurrant plantations. Scientific Horizons, 28(6), 65-76. https://doi.org/10.48077/scihor6.2025.65