Bio-ecological characteristics of Malus genus species in the context of prospective directions in horticulture and landscape forestry
Abstract
The study aimed to conduct a comparative assessment of representatives of the genus Malus based on morphological traits, biological characteristics, physiological mechanisms of resistance to fungal pathogens, ornamental value, and significance within the frameworks of horticulture, landscape forestry, and shelterbelt afforestation. Employing an integrative approach, the research examined the morphological traits and biological characteristics of domestic apple, European wild apple, Siberian crab apple, hybrid crab apple, and plum-leaved apple. Comparative analysis was carried out to evaluate the susceptibility and resistance of these species to scab pathogens and other diseases. The findings indicated that the domestic apple is characterised by large fruit size, with an average fruit mass of no less than 150 g. In contrast, the average fruit mass for Siberian crab apple, cherry crab apple, and hybrid crab apple does not exceed 0.7 g, 1.8 g, and 3.2 g, respectively. The authors have developed an integrated scale for the comparative evaluation of Malus species (or crab apple varieties) based on overall ornamental value. This scale included five criteria: crown appearance, leaf aesthetics, floral attractiveness, fruit visual appeal, and resistance to apple scab. For the first time, fruits of different Malus species have been analysed for seed productivity. Among these, crab apple varieties, particularly the European wild apple and the plumleaved apple, were identified as having the highest average number of seeds per fruit – 10 and 8 seeds, respectively. It was also shown that the seeds of the wild apple are relatively large, with the 100-seed weight exceeding 3.9 g. The study highlighted findings that confirm the ornamental value of certain Malus species, notably the Siberian crab apple and the hybrid crab apple, which received the highest overall ornamental scores of 4.5 and 5. This contrasts with the European wild apple, cherry apple, and domestic apple, which scored lower for ornamental attributes (3.2-3.9). It was noted that crab crab apple species such as Malus sylvestris (L.) Mill. and Malus baccata (L.) Borkh. are wellsuited for use in the creation of forest-park landscapes. Under the conditions of the Northern Forest-Steppe of Ukraine, Malus species were differentiated by their resistance to the scab pathogen (Venturia inaequalis Cooke). The results established that domestic apple (cultivar Dozhnytsia), hybrid crab apple, and plum-leaved apple exhibited high resistance to this pathogen, which affects both foliage and fruit. The practical application of these findings lies in enriching the informational database on Malus species with updated data, enabling their utilisation in various thematic studies
Keywords
Malus; morphology; plant physiology; resistance to phytopathogens; comparative evaluation
[1] Abbasi, P.A., Ali, S., Braun, G., Bevis, E., & Fillmore, S. (2019). Reducing apple scab and frogeye or black rot infections with salicylic acid or its analogue on field-established apple trees. Canadian Journal of Plant Pathology, 41(3), 345-354. doi: 10.1080/07060661.2019.1610070.
[2] Arabzada, A., & Sadigov, A. (2023). Decorative characteristics of introduced wild apple (Malus Mill) species in Absheron. Journal of ENDEMISM: Biodiversity & Environment, 1(1), 26-31. doi: 10.54414/MBPJ3111.
[3] Arnal, A., Lázaro, A., & Tardío, J. (2020). Morphological characterization of 23 Malus domestica Borkh cultivars from central Spain. Genetic Resources, 3(6), 22-37. doi: 10.46265/genresj.HJIF8839.
[4] Boldyzheva, L.D. (2020). Breeding for obtaining apple (Malus domestica Borkh.) immune cultivars. Horticulture, 75, 31-37. doi: 10.35205/0558-1125-2020-75-31-37.
[5] Bragard, C., et al. (2021). Commodity risk assessment of Malus domestica plants from Ukraine. EFSA Journal, 19(11), article number e06909. doi: 10.2903/j.efsa.2021.6909.
[6] Brite, E.B. (2021). The origins of the apple in Central Asia. Journal of World Prehistory, 34(2), 159-193. doi: 10.1007/ s10963-021-09154-8.
[7] Cebulj, A., Mikulič-Petkovšek, M., Veberič, R., & Jakopic, J. (2022). Effect of spring frost damage on apple fruit (Malus domestica Borkh.) inner quality at harvest. Agriculture, 12(1), article number 14. doi: 10.3390/ agriculture12010014.
[8] Chaploutskyi, A., & Butsyk, R. (2023). Formation of productivity of apple trees depending on the method and time of pruning of trees. Collected Works of Uman National University of Horticulture, 103(2), 69-76. doi: 10.32782/2415-8240-2023-103-1-69-76.
[9] Chen, X., et al. (2019). Sequencing of a wild apple (Malus baccata) genome unravels the differences between cultivated and wild apple species regarding disease resistance and cold tolerance. G3 (Bethesda), 9(7), 20512060. doi: 10.1534/g3.119.400245.
[10] Chen, Z., Yu, L., Liu, W., Zhang, J., Wang, N., & Chen, X. (2021). Research progress of fruit color development in apple (Malus domestica Borkh.). Plant Physiology and Biochemistry, 162, 267-279. doi: 10.1016/j.plaphy.2021.02.033.
[11] Convention on Biological Diversity. (1992, June). Retrieved from https://zakon.rada.gov.ua/laws/ show/995_030#Text.
[12] Convention on International Trade in Endangered Species of Wild Fauna and Flora. (1979, June). Retrieved from https://zakon.rada.gov.ua/laws/show/995_129#Text.
[13] Davies, T., Watts, S., McClure, K., Migicovsky, Z., & Myles, S. (2022). Phenotypic divergence between the cultivated apple (Malus domestica) and its primary wild progenitor (Malus sieversii). PLoS One, 17(3), article number e0250751. doi: 10.1371/journal.pone.0250751.
[14] FAO. (2023). Retrieved from https://www.fao.org/faostat/en/#da.
[15] Gómez-Candón, D., Mathieu, V., Martinez, S., Labbé, S., Delalande, M., & Regnard, J.-L. (2022). Unravelling the responses of different apple varieties to water constraints by continuous field thermal monitoring. Scientia Horticulturae, 299, article number 111013. doi: 10.1016/j.scienta.2022.111013.
[16] Greaves, E., & Husband, B. (2022). Impacts of hybridization on native crabapple (Malus coronaria) by domestic apple (Malus domestica) in Southern Ontario. Ontario: The University of Guelph.
[17] Guo, X., Zhang, D., & Bai, L. (2022). Development of EST-SSR markers and population genetic structure and genetic diversity of the Malus transitoria (Batalin) C. K. Schneider in Qinghai-Tibetan Plateau. Genetic Resources and Crop Evolution, 70, 1-15. doi: 10.1007/s10722-022-01477-5.
[18] Hardie, M., Oliver, G., Cotching, W., Walker, B., Lancaster, R., & Swarts, N. (2024). Health and characteristics of Australian apple growing soils. Applied and Environmental Soil Science, 2024, article number 9479986. doi: 10.1155/2024/9479986.
[19] Havryliuk, O., Kondratenko, T., Mazur, B., & Petrenko, D. (2023). Pollen quality and selection of pollinators ofcultivars of columnar type appleo. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 19(1). doi: 10.31548/dopovidi1(101).2023.005.
[20] Hulko, B.I. (2020). Fruit production. Workshop for the performance of practical work by students of the higher education level Bachelor of specialty 203 Horticulture and viticulture under the OPP Horticulture and viticulture. Dublyany: Lviv National University of Environmental Management.
[21] Ji, J., Li, Z., Tian, J., Zhang, J., Lu, Y., Qin, X., Li, J., Liu, L., Gao, Z., Hu, Y., & Yao, Y. (2021). ‘Hongbaleng’, a crabapple cultivar with large fruit and full-coverage red color. HortScience, 56(9), 1134-1138. doi: 10.21273/ HORTSCI15994-21.
[22] Khajuria, Y.P., Akhoon, B.A., Kaul, S., & Dhar., M.K. (2022). Secretomic Insights into the Pathophysiology of Venturia inaequalis: The causative agent of scab, a devastating apple tree disease. Pathogens, 12(1), article number 66. doi: 10.3390/pathogens12010066.
[23] Kondratenko, T.E., & Kuzminets, O.M. (2018). Pomology. Common and promising varieties of grain crops. Tutorial. Kyiv: Comprint.
[24] Kumar, A., Sharma, D.P., Kumar, P., Sharma, G., & Suprun, I.I. (2022). Comprehensive insights on Apple (Malus × domestica Borkh.) bud sport mutations and epigenetic regulations. Scientia Horticulturae, 297, article number 110979. doi: 10.1016/j.scienta.2022.110979.
[25] Kviklys, D., Viškelis, J., Liaudanskas, M., Janulis, V., Laužikė, K., Samuolienė, G., Uselis, N., & Lanauskas, J. (2022). Apple fruit growth and quality depend on the position in tree canopy. Plants, 11(2), article number 196. doi: 10.3390/plants11020196.
[26] Li, Z., et al. (2022). Chromosome-scale reference genome provides insights into the genetic origin and graftingmediated stress tolerance of Malus prunifolia. Plant Biotechnology Journal, 20(6), 1015-1017. doi: 10.1111/ pbi.13817.
[27] Liu, B., Zhang, C., Zhang, J., & Zhao, X. (2019). Wu Shan Shen Cha (Malus asiatica Nakai. Leaves)-derived flavonoids alleviate alcohol-induced gastric injury in mice via an anti-oxidative mechanism. Biomolecules, 9(5), article number 169. doi: 10.3390/biom9050169.
[28] Lou, G., Wang, S., Zhang, B., Cheng, Y., & Wanga, H. (2020). The complete chloroplast genome sequence of Malus sieboldii (Rosaceae) and its phylogenetic analysis. Mitochondrial DNA B Resources, 5(3), 2170-2171. doi: 10.1080/23802359.2020.1768940.
[29] Maharani, D., & Mursitama, T.N. (2023). Implementing Ethical ‘code of work ethics’: A case study of apple and foxconn supply chain. E3S Web of Conferences, 426, article number 02152. doi: 10.1051/e3sconf/202342602152.
[30] Mansfeld, B.N., Ou, S., Burchard, E., Yocca, A., Harkess, A., Gutierrez, B., Nocker, S.van, Tang, L., & Gottschalk, C. (2023). Genome of the North American wild apple species Malus angustifolia. bioRxiv. doi: 10.1101/2023.11.16.567428.
[31] Mbovora, S.M., Musvosvi, C., & Gasura, E. (2021). Morphological diversity among accessions of apple tree (Malus × Domestica Borkh). Advances in Agriculture, 2021, article number 7705856. doi: 10.1155/2021/7705856.
[32] Methodology of phytopathological studies for artificial infection of plants. (2017). Retrieved from https:// www.sops.gov.ua/uploads/page/5b7e70f017202.pdf.
[33] Mezhenskyj, V., & Mezhenska, L. (2021). Red flower apple in the collection of the National University of Life and Environmental Sciences of Ukraine. Ukrainian Journal of Forest and Wood Science, 12(4), 72-82. doi: 10.31548/ forest2021.04.007.
[34] Nabi, S.U., et al. (2023). Tissue and time optimization for real-time detection of apple mosaic virus and apple necrotic mosaic virus associated with mosaic disease of apple (Malus domestica). Viruses, 15(3), article number 795. doi: 10.3390/v15030795.
[35] Olivares, A.A., Gogorcena, Y., Javier, G., Javier, T., & Almudena, L. (2020). Simple sequence repeat characterisation of traditional apple cultivars (Malus domestica Borkh.) grown in the region of Madrid (Central Spain). Plant Molecular Biology Reporter, 38(4). 676-690. doi: 10.1007/s11105-020-01240-z.
[36] Reig, G., Lordan, J., Sazo, M.M., Hoying, S., Fargione, M., Reginato, G., Donahue, D.J., Francescatto, P., Fazio, G., & Robinson, T. (2019). Long-term performance of ‘Gala’, Fuji’ and ‘Honeycrisp’ apple trees grafted on Geneva® rootstocks and trained to four production systems under New York State climatic conditions. Scientia Horticulturae, 244, 277-293. doi: 10.1016/j.scienta.2018.09.025.
[37] Ruhsam, M., Bell, D., Hart, M., & Hollingsworth, P. (2022) The genome sequence of the European crab apple, Malus sylvestris (L.) Mill., 1768. Wellcome Open Research, 7, article number 296. doi: 10.12688/ wellcomeopenres.18645.1.
[38] Spengler, R.N. (2019). Origins of the apple: The role of Megafaunal Mutualism in the domestication of Malus and Rosaceous Trees. Front Plant Science, 10, article number 617. doi: 10.3389/fpls.2019.00617.
[39] Tkachuk, S.O. (2016). Methodology for examination of varieties of fruit, berry, nut and grape plant varieties for distinction, homogeneity and stability. Vinnytsia: PE D. Korzun.
[40] Voloshina, V.V., & Gomenyuk, V.I. (2021). Genetic diversity of Malus Mill. in the collection plantings of the Experimental pomology station named after L.P. Symyrenko. Journal of Native and Alien Plant Studies, 1, 36-39. doi: 10.37555/2707-3114.1.2021.247356.
[41] Walas, Ł., Alipour, S., Haq S.M., & Alamri, S. (2024). The potential range of west Asian apple species Malus orientalis Uglitzk. under climate change. BMC Plant Biology, 24, article number 381. doi: 10.1186/s12870-02405081-w.
[42] Woodall, C., Liknes, G.C., Bunker, J.P., Peace, C.P., & Frank, J.M. (2023). Forgotten forest relics: Apple trees (Malus spp.) in eastern U.S. forests. Biodiversity and Conservation, 32, 1657-1670. doi: 10.1007/s10531-023-02569-z.