THE EFFECT OF «ENZYMNYI» DETERGENT ONTO MICROBIAL BIOFILMS ENTEROCOCCUS FАECALIS AND ESCHERIСHIA COLI

Poor sanitation of dairy processing equipment results in milk residue on the surface that becomes a perfect nutrient medium for microbial development. To fight Enterococcus faecalis and Escherishia coli formed in biofilm, we developed a liquid enzyme detergent "ENZYMNYI" capable of destroying extracellular polysaccharide matrix. Previous research showed that the most optimal proteolytic activity manifested at the temperature of working solution of 60°C, pH 8.35 and water hardness 0.35-0.714 mEq/l. Optical density of microbial biofilms of Enterococcus faecalis and Escherishia coli decreases with increasing temperature of an enzyme product and continuity of its effect leading to destruction of biofilms. The enzyme product concentration of 0,01% at the temperature 60°C and duration of 60 min. was the most active to biofilm microorganisms, optical density of biofilms being considered low