Biochemical composition of sweet cherry leaves depending on the method of soil maintenance in an organic gardes
Abstract
Conducting sustainable agriculture involves not only increasing the productivity of crops and increasing the volume of crop production, but also preserving ecosystems. Mulching the soil in orchards is one of the ways to preserve the natural balance of agricultural landscapes. But the effect of competition with grasses on the biochemical composition of fruit tree tissues has not yet been definitively elucidated. The purpose of this study was to determine the influence of soil retention under mulching (compared to pure steam) on the biochemical composition of cherry leaf tissues. The research was conducted in an organic cherry orchard (Prunus avium L. / Prunus mahaleb) during 2017-2019 in the conditions of the Southern Steppe of Ukraine. A significant decrease in the content of ascorbate, glutathione, sugars, total reducing activity and activity of antioxidant enzymes was established under conditions of prolonged drought and an increase in the content of titrated acids. The total reducing activity under mulching conditions tended to increase. A gradual increase in the content of titrated acids, ascorbate, sugars, phenolic substances, and glutathione was recorded in the leaves of cherry trees from the flowering phase to November. In the autumn phase, a significant increase in sugars and phenolic substances was established in cherry leaves under the conditions of mulching in 2017, and in 2018 – phenolic substances; in 2019 – phenolic substances and ascorbate. An increase in the content of malondialdehyde (MDA) and the activity of antioxidant enzymes was found during the growing season of cherries in both variants of the experiment. In the November phase only in 2019, the MDA content was significantly higher by 14% under the condition of mulching. Under mulching conditions, a significant increase in ascorbate peroxidase (by 28-30%) and polyphenol oxidase (by 45-46%) was determined. In 2018 and 2019, a 2.4-fold increase in peroxidase activity in cherry leaves was determined. Research results help to understand the mechanisms of adaptation of fruit plants to stress factors (drought, competition with natural grasses) and can be used as an argument in favour of mulching in organic cherry orchards
Keywords
mulching, vitamin C, glutathione, titrated acids, catalase, ascorbate peroxidase, polyphenol oxidase
[1] Afonso, S., Oliveira, I.V., Meyer, A.S., Aires, A., Saavedra, M.J., & Gonçalves, B. (2020). Phenolic profile and bioactive potential of stems and seed kernels of sweet cherry fruit. Antioxidants, 9(12), article number 1295. doi: 10.3390/antiox9121295.
[2] Anjum, N.A., Umar, S., & Chan, M.T. (2010). Ascorbate-glutathione pathway and stress tolerance in plants. Dordrecht: Science + Business Media B.V. doi: 10.1007/978-90-481-9404-9.
[3] Atucha, A., Merwin, I.A., & Brown, M.G. (2011). Long-term effects of four groundcover management systems in an apple orchard. Horticulture Science, 46(8), 1176-1183. doi: 10.21273/HORTSCI.46.8.1176.
[4] Balestrini, R. Chitarra, W., Antoniou, C., Ruocco, M., & Fotopoulos, V. (2018). Improvement of plant performance under water deficit with the employment of biological and chemical priming agents. Journal of Agricultural Science, 156, 680-688. doi: 10.1017/S0021859618000126.
[5] Bastos, C., Barros, L., Dueñas, M., Calhelha, R.C., Queiroz, M.J.R.P., Santos-Buelga, C. & Ferreira, I.C.F.R. (2015). Chemical characterisation and bioactive properties of Prunus avium L.: The widely studied fruits and the unexplored stems. Food Chemistry, 173, 1045-1053. doi: 10.1016/j.foodchem.2014.10.145.
[6] Bonyanpour, A.R., & Jamali, B. (2020). Seasonal enzymatic and nonenzymatic antioxidant responses in seven Iranian pomegranate cultivars. Advances in Horticultural Science, 34(3), 265-276. doi: 10.13128/ahsc8283.
[7] Cai, B., Wang, H., Liu, T., Zhuang, W., Wang, Z., Qu, S.C., & Qin, Y.L. (2019). Effects of gibberellins A4 on budbreak, antioxidant enzymes’ activity and proline content of flower buds in sweet cherry (Prunus avium). Acta Physiologiae Plantarum, 41, article number 88. doi: 10.1007/s11738-019-2876-z.
[8] Cheynier, V., Comte, G., Davies, K.M., Lattanzio, V., & Martens, S. (2013). Plant phenolics: Recent advances on their biosynthesis, genetics, and ecophysiology. Plant Physiology and Biochemistry, 72, 1-20. doi: 10.1016/j.plaphy.2013.05.009.
[9] Costa, H., Gallego, S.M., & Tomaro, M.L. (2002). Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Science, 162(6), 939-945. doi: 10.1016/S0168-9452(02)00051-1.
[10] Djilas, S., Čanadanović-Brunet, J., & Ćetković, G. (2009). By-products of fruits processing as a source of phytochemicals. Chemical Industry & Chemical Engineering Quarterly, 15, 191-202. doi: 10.2298/CICEQ0904191D.
[11] Dziadek, K., Kopeć, A., & Czaplicki, S. (2018). The petioles and leaves of sweet cherry (Prunus avium L.) as a potential source of natural bioactive compounds. European Food Research Technology, 244(8), 1415-1426. doi: 10.1007/s00217-018-3055-y.
[12] Dziadek, K., Kopeć, A., & Tabaszewska, M. (2019). Potential of sweet cherry (Prunus avium L.) by-products: bioactive compounds and antioxidant activity of leaves and petioles. European Food Research Technology, 245, 763-772. doi: 10.1007/s00217-018-3198-x.
[13] Esitken, A. (2011). Use of plant growth promoting rhizobacteria in horticultural crops. In D.K. Maheshwari (Ed.), Bacteria in agrobiology: Crop ecosystems (pp.189-235). doi: 10.1007/978-3-642-18357-7_8.
[14] Frew, J.E., Jones, P., & Sholes, G. (1983). Spectrophotometric determination of hydrogen peroxide and organic hydropheroxides at low concentrations in aqueous solution. Analytica Chimica Acta, 155, 139-146. doi: 10.1016/S0003-2670(00)85587-7.
[15] Gerasko, T., Pyda, S., & Ivanova, I. (2021). Effect of living mulch on soil conditions and morphometrical indices of sweet cherry trees. International Journal of Applied Agricultural Sciences, 7(1), 50-56. doi: 10.11648/j. ijaas.20210701.14.
[16] Gest, N., Gautier, H., & Stevens, R. (2012). Ascorbate as seen through plant evolution: The rise of a successful molecule. Journal of Experimental Botany, 2012, 64(1), 33-53. doi: 10.1093/jxb/ers297.
[17] Gill, S.S., Anjum, N.A., Hasanuzzaman, M., Gill, R., Trivedi, D.K., Ahmad, I., Pereira, E., & Tuteja, N. (2013). Glutathione and glutathione reductase: A boon in disguise for plant abiotic stress defense operations. Plant Physiology and Biochemistry, 70, 204-212. doi: 10.1016/j.plaphy.2013.05.032.
[18] Gorodniy, M.M. (Ed). (2006). Applied biochemistry and quality management of crop products: Textbook. Kyiv: Aristei.
[19] Grebennikova, O.A. (2011). Biologically active speech in fruits, leaves and processing products of promising varieties in alichi. (Candidate thesis, Odesa I.I. Mechnikov National University, Odesa, Ukraine).
[20] Hallmann, E., & Sabała, P. (2020). Organic and conventional herbs quality reflected by their antioxidant compounds concentration. Applied Science, 10, article number 3468. doi: 10.3390/app10103468.
[21] Ivanova, I., Serdyuk, M., Kryvonos, I., Yeremenko, O., & Тymoshuk, Т. (2020). Formation of flavoring qualities of sweet cherry fruits under the influence of weather factors. Scientific Horizons, 4(89), 72-81.
[22] Ivanova, I., Serdyuk, M., Malkina, V., Priss, О., Herasko, T., & Тymoshchuk, Т. (2021). Investigation into sugars accumulation in sweet cherry fruits under abiotic factors effects. Agronomy Research, 19(2), 44-457. doi: 10.15159/ar.21.004.
[23] Ivanova, І., Serdyuk, М., Malkina, V., Tymoshchuk, T., Vorovka, M., Mrynskyi, I., & Adamovych, A. (2022). Studies of the impact of environmental conditions and varietal features of sweet cherry on the accumulation of vitamin C in fruits by using the regression analysis method. Acta Agriculturae Slovenica, 118(2), 1-12. doi: 10.14720/aas.2022.118.2.2404.
[24] Jesus, F., Gonçalves, A.C., Alves, G., & Silva, L.R. (2019). Exploring the phenolic profile, antioxidant, antidiabetic and anti-hemolytic potential of Prunus avium vegetal parts. Food Research International, 116, 600-610. doi: 10.1016/j.foodres.2018.08.079.
[25] Mateos-Fierro, Z., Fountain, M.T., Garratt, M.P.D., & Ashbrook, K. (2021). Active management of wildflower strips in commercial sweet cherry orchards enhances natural enemies and pest regulation services. Agriculture, Ecosystems & Environment, 317, article number 107485. doi: 10.1016/j.agee.2021.107485.
[26] Methods of qualification examination of plant varieties for suitability for distribution in Ukraine. Methods for determining the quality of crop products. (n.d.). Retrieved from http://www.minagro.gov.ua/.
[27] Oszmiański, J., Wojdyło, A., Lachowicz, S., Gorzelany, J., & Matłok, N. (2016). Comparison of bioactive potential of cranberry fruit and fruit-based products versus leaves. Journal of Functional Foods, 22, 232-242. doi: 10.1016/j.jff.2016.01.015.
[28] Pereira, S., Silva, V., Bacelar, E., Guedes, F., Silva, A.P., Ribeiro, C., & Gonçalves, B. (2020). Cracking in sweet cherry cultivars early bigi and lapins: Correlation with quality attributes. Plants, 9, article number 1557. doi: 10.3390/plants9111557.
[29] Picariello, G., De Vito, V., Ferranti, P., Paolucci, M., & Volpe, M.G. (2016). Species- and cultivardependent traits of Prunus avium and Prunus cerasus polyphenols. Journal Food Composition and Analysis, 40, 50-57. doi: 10.1016/j.jfca.2015.10.002.
[30] Polonskaya, A.K., Ezhov, V.N., Korniliev, G.V., & Grebennikova, O.A. (2007). Biologically active substances of the leaves of some fruit crops in connection with the prospect of their use in food. Scientific Notes of the Tavrichesky National University named after Vernadsky. Series “Biology, Chemistry”, 20(59), 122-127.
[31] Prvulović, D., Popović., M, Malenčić, Đ., & Ljubojevic, M. (2011). Phenolic compounds in sweet cherry (Prunus avium L.) petioles and their antioxidant properties. Research Journal of Agricultural Science, 43, 198-202
[32] Richter, A.A. (2001). Improving the quality of fruits of southern cultures. Simferopol: Tavria.
[33] Sharma, P., Jha, A., Dubey, R.S., & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012, 1-26. doi: 10.1155/2012/217037.
[34] Szpadzik, E., Krupa, T., Wojciech, N., & Jadczuk-Tobjasz, E. (2019). Yielding and fruit quality of selected sweet cherry (Prunus avium) cultivars in the conditions of central Poland. Acta Scientiarum Polonorum Hortorum Cultus, 18(3), 117-126. doi: 10.24326/asphc.2019.3.11.
[35] Tabart, J., Kevers, C, Pincemail, J., Defraigne, J.-O., & Dommes, J. (2006). Antioxidant capacity of black currant varies with organs, season and cultivars. Journal Agricultural Food Chemistry, 54, 6271-6276. doi: 10.1021/jf061112y.
[36] Teleszko, M., & Wojdyło, A. (2015). Comparison of phenolic compounds and antioxidant potential between selected edible fruits and their leaves. Journal of Functional Foods, 14, 736-746. doi: 10.1016/j.jff.2015.02.041.
[37] Turrini, A., Caruso, G., Avio, L., & Gennai, C. (2017). Protective green cover enhances soil respiration and native mycorrhizal potential compared with soil tillage in a high-density olive orchard in a long term study. Applied Soil Ecology, 116, 70-78. doi: 10.1016/j.apsoil.2017.04.001.
[38] Uysal, S. (2020). Comparative antioxidant capacity and enzyme inhibitory effect of extracts from Prunus avium leaves. Kastamonu University Journal of Forestry Faculty, 20(3), 234-242. doi: 10.17475/kastorman.849538.
[39] Walker, R.P., Benincasa, P., Battistelli, A., Moscatello, S., Técsi, L., Leegood, R.C., & Famiani, F. (2018). Gluconeogenesis and nitrogen metabolism in maize. Plant Physiology Biochemistry, 130, 324-333. doi: 10.1016/j.plaphy.2018.07.009.
[40] Waterhouse, A.L. (2002). Polyphenolics: Determinaiton of total phenolics. In R.E. Wrolstad (Ed.), Current protocols in food analytical chemistry (pp. 1111-1118). New York: John Wiley & Sons.
[41] Yao, S.R., Merwin, I.A., Bird, G.W., Abawi, G.S., & Thies, J.E. (2005). Orchard floor management practices that maintain vegetative or biomass groundcover stimulate soil microbial activity and alter soil microbial community composition. Plant Soil, 271(1/2), 377-389. doi: 10.1007/s11104-004-3610-0.
[42] Yüksekkaya, Ş., Bülent, B., Sağlam, H., Pekmez, H., Cansu, Ü., Karaaslan, A., & Karaaslan, M. (2021). Valorization of fruit processing by-products: Free, esterified and insoluble bound phytochemical extraction from cherry (Prunus avium) tissues and their biological activities. Journal of Food Measurement & Characterization, 15(2), 1092-1107. doi: 10.1007/s11694-020-00698-5.