Identification of the bioaerosol microbiota in veterinary clinics as the key to preventing nosocomial infection
Abstract
Nosocomial infections are important in veterinary medicine clinics, as they can contaminate surfaces and infect animals through bioaerosol and are the cause of ineffective antibiotic therapy. This paper presents a study of the effect of disinfection on the microbiota of bioaerozoic veterinary clinics. The purpose of this study was to establish the composition of the bioaerosol microbiota in veterinary clinic premises before and after disinfection with ultraviolet bactericidal lamps. Bioaerosol samples were taken in the premises of veterinary clinics by sedimentation method. Identification of the isolated microbiota was performed using classical methods involving commercial test systems for identifying microorganisms. It was established that the permanent microbiota of the bioaerosol of veterinary clinics includes the following representatives of gram-positive genera: Staphylococcus (coagulase-negative species), Streptococcus spp., Micrococcus spp., and Corynebacterium spp. These bacterial genera were present in the bioaerosol of all rooms in 100% of cases. Gram-negative species of bacteria were found in small quantities in the bioaerosol of such rooms as for the primary examination and the manipulation area with boxes for keeping sick animals. Representatives of gram-negative species were detected in a considerably larger number from the bioaerosol of the dental operating room during the day of the clinic. After disinfection with bactericidal lamps, pathogens of nosocomial infections (S. aureus, S. pseudintermedius, Acinetobacter baumani, P. aeruginosa) were released in the bioaerosol of such rooms as the primary examination, the manipulation area with boxes for keeping sick animals, and the dental operating room. This indicates that bioaerosol can serve as a medium for the spread of nosocomial infections among animals in veterinary clinics. Thus, conducting microbiological monitoring of bioaerosol in veterinary clinics will allow identifying pathogens of nosocomial infections and introducing preventive measures for the spread of pathogens
Keywords
air microflora, disinfection with ultraviolet lamps, nosocomial pathogens, pathogenic bacteria
[1] Berhilevych, O., Kasianchuk, V., Kukhtyn, M., Shubin, P., & Butsyk, A. (2021). Comparison of cell sizes of methicillin-resistant Staphylococcus aureus with Presence and absence of the MecA Gene. Microbiological Journal, 83(1), 68-77. doi: 10.15407/microbiolj83.01.068.
[2] Chai, M.H., Sukiman, M.Z., Liew, Y.W., Shapawi, M.S., Roslan, F.S., Hashim, S.N., & Ghazali, M.F. (2021). Detection, molecular characterization, and antibiogram of multi-drug resistant and methicillin-resistant Staphylococcus aureus (MRSA) isolated from pets and pet owners in Malaysia. Iranian Journal of Veterinary Research, 22(4), 277-287. doi: 10.22099/ijvr.2021.39586.5752.
[3] Chen, P., Guo, X., & Li, F. (2022). Antibiotic resistance genes in bioaerosols: Emerging, non-ignorable and pernicious pollutants. Journal of Cleaner Production, 348, article number 131094. doi: 10.1016/j.jclepro.2022.131094.
[4] Chueahiran, S., Yindee, J., Boonkham, P., Suanpairintr, N., & Chanchaithong, P. (2021). Methicillin-resistant Staphylococcus aureus clonal complex 398 as a major MRSA lineage in dogs and cats in Thailand. Antibiotics, 10(3), article number 243. doi: 10.3390/antibiotics10030243.
[5] Elnageh, H.R., Hiblu, M.A., Abbassi, M.S., Abouzeed, Y.M., & Ahmed, M.O. (2020). Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs. Open Veterinary Journal, 10(4), 452-456. doi: 10.4314/ovj.v10i4.13.
[6] Fahlgren, C., Hagström, A., Nilsson, D., & Zweifel, U.L. (2010). Annual variations in the diversity, viability, and origin of airborne bacteria. Applied and Environmental Microbiology, 76(9), 3015-3025. doi: 10.1128/aem.02092-09.
[7] Feßler, A.T., Schuenemann, R., Kadlec, K., Hensel, V., Brombach, J., Murugaiyan, J., Oechtering, G., Burgener, I.A., & Schwarz, S. (2018). Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) among employees and in the environment of a small animal hospital. Veterinary Microbiology, 221, 153-158. doi: 10.1016/j.vetmic.2018.06.001.
[8] Giannouli, M., Antunes, L.C., Marchetti, V., Triassi, M., Visca, P., & Zarrilli, R. (2013). Virulence-related traits of epidemic Acinetobacter baumannii strains belonging to the international clonal lineages I-III and to the emerging genotypes ST25 and ST78. BMC Infectious Diseases, 13(1), article number 282. doi: 10.1186/1471-2334-13-282.
[9] Hamido, A.J., Sirika, N.B., & Omar, I.A. (2022). Literature review on antibiotics. Clinical Medicine and Health Research Journal, 2(4), 174-182. doi: 10.18535/cmhrj.v2i4.65.
[10] Horiuk, Y., Kukhtyn, M., Horiuk, V., Kernychnyi, S., & Tarasenko, L. (2020). Characteristics of bacteriophages of the Staphylococcus aureus variant bovis. Veterinary Medicine-Czech, 65, 421-426. doi: 10.17221/55/2020-VETMED.
[11] Hritcu, O.M., Schmidt, V.M., Salem, S.E., Maciuca, I.E., Moraru, R.F., Lipovan, I., & Timofte, D. (2020). Geographical variations in virulence factors and antimicrobial resistance amongst staphylococci isolated from dogs from the United Kingdom and Romania. Frontiers in Veterinary Science, 7, 1-10. doi: 10.3389/fvets.2020.00414.
[12] Jeong, S.B., Ko, H.S., Heo, K.J., Shin, J.H., & Jung, J.H. (2022). Size distribution and concentration of indoor culturable bacterial and fungal bioaerosols. Atmospheric Environment: X, 15, article number 100182. doi: 10.1016/j.aeaoa.2022.100182.
[13] Kempf, M., & Rolain, J.-M. (2012). Emergence of resistance to carbapenems in Acinetobacter baumannii in Europe: Clinical impact and therapeutic options. International Journal of Antimicrobial Agents, 39(2), 105-114. doi: 10.1016/j.ijantimicag.2011.10.004.
[14] Kisera, Y., Bozhyk, L., Grynevych, N., & Martyniv, Y. (2021). Species composition of circulation microflora and its resistance to antibacterial drugs in the conditions of the impulse veterinary clinic of the city of Lviv. Scientific Bulletin of Veterinary Medicine, 2(168), 65-71. doi: 10.33245/2310-4902-2021-168-2-65-71.
[15] Krapf, M., Müller, E., Reissig, A., Slickers, P., Braun, S.D., Müller, E., & Monecke, S. (2019). Molecular characterisation of methicillin-resistant Staphylococcus pseudintermedius from dogs and the description of their SCCmec elements. Veterinary Microbiology, 233, 196-203. doi: 10.1016/j.vetmic.2019.04.002.
[16] Lee, G., & Yoo, K. (2022). A review of the emergence of antibiotic resistance in bioaerosols and its monitoring methods. Reviews in Environmental Science and Biotechnology, 21, 799-27. doi: 10.1007/s11157-022-09622-3.
[17] Loncaric, I., Lepuschitz, S., Ruppitsch, W., Trstan, A., Andreadis, T., Bouchlis, N., & Spergser, J. (2019). Increased genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) isolated from companion animals. Veterinary Microbiology, 235, 118-126. doi: 10.1016/j.vetmic.2019.06.013.
[18] Mocherniuk, M.M., Kukhtyn, M.D., Horiuk, Y.V., Horiuk, V.V., Tsvigun, O.A., & Tokarchuk, T.S. (2022). Microflora of boxes for holding veterinary patients in clinics. Regulatory Mechanisms in Biosystems, 13(3), 257-264. doi: 10.15421/022233.
[19] Morgado-Gamero, W.B., Parody, A., Medina, J., Rodriguez-Villamizar, L.A., & Agudelo-Castañeda, D. (2021). Multi-antibiotic resistant bacteria in landfill bioaerosols: Environmental conditions and biological risk assessment. Environmental Pollution, 290, article number 118037. doi: 10.1016/j.envpol.2021.118037.
[20] Murray, A.K., Lee, J., Bendall, R., Zhang, L., Sunde, M., Schau Slettemeås, J., Gaze, W., Page, A.J., & Vos, M. (2018). Staphylococcus cornubiensis sp. nov., a member of the Staphylococcus intermedius Group (SIG). International Journal of Systematic and Evolutionary Microbiology, 68(11), 3404-3408. doi: 10.1099/ijsem.0.002992.
[21] Naziri, Z., Poormaleknia, M., & Ghaedi Oliyaei, A. (2022). Risk of sharing resistant bacteria and/or resistance elements between dogs and their owners. BMC Veterinary Research, 18(1), 1-8. doi: 10.1186/s12917-022-03298-1.
[22] Pertegal, V., Lacasa, E., Cañizares, P., Rodrigo, M.A., & Sáez, C. (2022). Understanding the influence of the bioaerosol source on the distribution of airborne bacteria in hospital indoor air. Environmental Research, 216(1), article number 114458. doi: 10.1016/j.envres.2022.114458.
[23] Sellera, F.P., Da Silva, L.C., & Lincopan, N. (2021). Rapid spread of critical priority carbapenemase-producing pathogens in companion animals: A one health challenge for a post-pandemic world. Journal of Antimicrobial Chemotherapy, 76(9), 2225-2229. doi: 10.1093/jac/dkab169.
[24] Sitkowska, J., Sitkowski, W., Sitkowski, L., Lutnicki, K., Adamek, L., & Wilkolek, P. (2015). Seasonal microbiological quality of air in veterinary practices in Poland. Annals of Agricultural and Environmental Medicine, 22(4), 614-624. doi: 10.5604/12321966.1185763.
[25] Smith, A., Wayne, A.S., Fellman, C.L., & Rosenbaum, M.H. (2019). Usage patterns of carbapenem antimicrobials in dogs and cats at a veterinary tertiary care hospital. Journal of Veterinary Internal Medicine, 33(4), 1677-1685. doi: 10.1111/jvim.15522.
[26] Song, L., Wang, C., Jiang, G., Ma, J., Li, Y., Chen, H., & Guo, J. (2021). Bioaerosol is an important transmission route of antibiotic resistance genes in pig farms. Environment International, 154, article number 106559. doi: 10.1016/j.envint.2021.106559.
[27] Tamakan, H., & Gocmen, H. (2022). Genetic characterization of methicillin resistant Staphylococcus pseudintermedius in dogs and cats in Cyprus: Comparison of MRSP and MRSA results. Pakistan Journal of Zoology, 54(4).
[28] Tsay, M.D., Tseng, C.C., Wu, N.X., & Lai, C.Y. (2020). Size distribution and antibiotic-resistant characteristics of bacterial bioaerosol in intensive care unit before and during visits to patients. Environment International, 144, article number 106024. doi: 10.1016/j.envint.2020.106024.
[29] Vos, P., Garrity, G., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., & Whitman, W.B. (Eds.). (2011). Bergey's manual of systematic bacteriology. New York: Springer Science & Business Media. doi: 10.1007/b92997.
[30] Zheng, Y., Dong, H., Wang, S., Zhang, Y., & Cong, Q. (2023). A new air cleaning technology to synergistically reduce odor and bioaerosol emissions from livestock houses. Agriculture, Ecosystems & Environment, 342, article number 108221. doi: 10.1016/j.agee.2022.108221.